Reduced Nicotinamide Adenine Dinucleotide Phosphate–Diaphorase Histochemistry: Light and Electron Microscopic Investigations
作者： Kiminao Mizukawa
DOI: 10.1016/B978-0-12-185255-9.50031-6
关键词:
摘要: Publisher Summary This chapter discusses several modifications of the procedures for nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase histochemistry and distribution these NADPH-diaphorase–positive cell groups nerve terminals in central nervous system. It also ultrastructral localization NADPH-diaphorase coexistence other neuropeptides positive neurons fibers. The best procedure involves washing out blood proper fixation. further presents histochemical methods. One methods is a direct method using NADPH, two are indirect NADPH. Typical intensity found cerebral cortex, striatum, accumbens, pedunculopontine tegmental, parabrachial, pontine reticular nuclei mammalian brains. Several subtypes can be classified by their characteristic profiles. classification presented chapter. Under electron microscope, show large amounts electron-dense reaction product formazan, which not confined to any particular subcellular organelle.
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