Characterization of the Lipidomic Profile of Human Coronavirus-Infected Cells: Implications for Lipid Metabolism Remodeling upon Coronavirus Replication

摘要: Lipids play numerous indispensable cellular functions and are involved in multiple steps the replication cycle of viruses. Infections by human-pathogenic coronaviruses result diverse clinical outcomes, ranging from self-limiting flu-like symptoms to severe pneumonia with extrapulmonary manifestations. Understanding how lipids may modulate pathogenicity remains poor. To this end, we utilized human coronavirus 229E (HCoV-229E) as a model comprehensively characterize host cell lipid response upon infection an ultra-high performance liquid chromatography-mass spectrometry (UPLC–MS)-based lipidomics approach. Our results revealed that glycerophospholipids fatty acids (FAs) were significantly elevated HCoV-229E-infected cells linoleic acid (LA) arachidonic (AA) metabolism axis was markedly perturbed HCoV-229E infection. Interestingly, exogenous supplement LA or AA suppressed virus replication. Importantly, inhibitory effect on also conserved for highly pathogenic Middle East respiratory syndrome (MERS-CoV). Taken together, our study demonstrated metabolic remodeling associated propagation. data further suggested regulation would be common druggable target infections.