Drug target validation and identification of secondary drug target effects using DNA microarrays

摘要: We describe here a method for drug target validation and identification of secondary tar- get effects based on genome-wide gene expression patterns. The is demonstrated by several experiments, including treatment yeast mutant strains defective in calcineurin, im- munophilins or other genes with the immunosuppressants cyclosporin A FK506. Presence absence characteristic 'signature' pattern altered drug-treated cells mutation encoding putative established whether that was required to generate signature. Drug dependent were seen 'targetless' cells, showing FK506 affects additional pathways independent calcineurin munophilins. described permits direct confirmation targets recog- nition drug-dependent changes are modulated through distinct from drug's intended target. Such may prove useful improving effi- ciency development programs.