The Rat and Mouse Homologues of MASP-2 and MAp19, components of the Lectin Activation Pathway of Complement
作者： Wilhelm J. Schwaeble , Cordula M. Stover , Steffen Thiel , Nicholas J. Lynch
DOI:
关键词:
摘要: Recently, we described two novel constituents of the multimolecular initiation complex mannan-binding lectin (MBL) pathway complement activation, a serine protease 76 kDa, termed MASP-2, and MASP-2 related plasma protein 19 MAp19. Upon activation MBL/MASPs/MAp19 complex, cleaves fourth component C4, while role MAp19 within MBL/MASP-1/MASP-2/MAp19 remains to be clarified. In humans, mRNA species encoding (2.6 kb) (1.0 arise by an alternative polyadenylation/splicing mechanism from single structural gene. Here, report complete primary structures rat homologue mouse We show that both are part MBL demonstrate their exclusively hepatic biosynthesis. Southern blot PCR analyses genomic DNA indicate as in encoded
jimmunol.org 参考文章(45)
Mario Tosi, Christiane Duponchel, Tommaso Meo, Evelyne Couture-Tosi, Complement genes C1r and C1s feature an intronless serine protease domain closely related to haptoglobin. Journal of Molecular Biology. ,vol. 208, pp. 709- 714 ,(1989) , 10.1016/0022-2836(89)90161-7
Minoru Takahashi, Yuichi Endo, Teizo Fujita, Misao Matsushita, A truncated form of mannose-binding lectin-associated serine protease (MASP)-2 expressed by alternative polyadenylation is a component of the lectin complement pathway International Immunology. ,vol. 11, pp. 859- 863 ,(1999) , 10.1093/INTIMM/11.5.859
H.-X. Liu, M. Zhang, A. R. Krainer, Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins Genes & Development. ,vol. 12, pp. 1998- 2012 ,(1998) , 10.1101/GAD.12.13.1998
M McKeown, Alternative mRNA Splicing Annual Review of Cell Biology. ,vol. 8, pp. 133- 155 ,(1992) , 10.1146/ANNUREV.CB.08.110192.001025
M Niwa, S D Rose, S M Berget, In vitro polyadenylation is stimulated by the presence of an upstream intron. Genes & Development. ,vol. 4, pp. 1552- 1559 ,(1990) , 10.1101/GAD.4.9.1552
Kristin K Nelson, Michael R Green, Mammalian U2 snRNP has a sequence-specific RNA-binding activity. Genes & Development. ,vol. 3, pp. 1562- 1571 ,(1989) , 10.1101/GAD.3.10.1562
Michael R. Green, Biochemical Mechanisms of Constitutive and Regulated Pre-mRNA Splicing Annual Review of Cell Biology. ,vol. 7, pp. 559- 599 ,(1991) , 10.1146/ANNUREV.CB.07.110191.003015
LB Smillie, BS Hartley, Histidine sequences in the active centres of some 'serine' proteinases. Biochemical Journal. ,vol. 101, pp. 232- 241 ,(1966) , 10.1042/BJ1010232
Tom Maniatis, Joseph Sambrook, E. F. Fritsch, Molecular Cloning: A Laboratory Manual ,(2001)
Motoo Kimura, The neutral theory of molecular evolution and the world view of the neutralists. Genome. ,vol. 31, pp. 24- 31 ,(1989) , 10.1139/G89-009