In Vitro Evaluation of Anti-Colon Cancer Potential of Crude Extracts of Fucoidan Obtained from Sargassum Glaucescens Pretreated by Compressional-Puffing
作者： Wei-Cheng Shiao , Chia-Hung Kuo , Yung-Hsiang Tsai , Shu-Ling Hsieh , Ai-Wei Kuan
DOI: 10.3390/APP10093058
关键词:
摘要: Fucoidans constitute a family of fucose-rich sulfated polysaccharides, which possess multiple characteristics, including antioxidant, antitumor, antivirus, anticoagulant, and anti-inflammatory properties. In addition, the incidence colon cancer has risen rapidly worldwide. present study, fucoidan extracts were extracted from Sargassum glaucescens (SG) pretreated by compressional-puffing, four fucoidans (SG1-SG4) obtained with different puffing conditions. It was found that SG4 possessed highest extraction yield, relatively high cytotoxicity against human carcinoma HT-29 cells, low to normal as compared other fucoidans. Moreover, caused cell cycle arrest cells at sub-G1, S, G2/M phases. also induced cellular apoptosis, evidenced loss mitochondrial membrane potential (MMP), increased cytochrome c release, activation caspase-9 -3, DNA fragmentation, early late apoptotic visualized annexin V/propidium iodide (PI) assay. Additional biological experiments revealed Akt/mammalian target rapamycin (mTOR)/S6 pathway is involved in SG4-induced apoptosis cells. These results clearly indicate showed anti-colon via induction thus may have possible application an adjuvant therapeutic agent treatment.
scilit.net参考文章(37)
Prosenjit Sen, Sebanti Mukherjee, Doel Ray, Sanghamitra Raha, Involvement of the Akt/PKB signaling pathway with disease processes. Molecular and Cellular Biochemistry. ,vol. 253, pp. 241- 246 ,(2003) , 10.1023/A:1026020101379
Cheng-Yuan Wang, Tien-Chou Wu, Shu-Ling Hsieh, Yung-Hsiang Tsai, Chia-Wen Yeh, Chun-Yung Huang, Antioxidant activity and growth inhibition of human colon cancer cells by crude and purified fucoidan preparations extracted from Sargassum cristaefolium. Journal of Food and Drug Analysis. ,vol. 23, pp. 766- 777 ,(2015) , 10.1016/J.JFDA.2015.07.002
Martin CROMPTON, The mitochondrial permeability transition pore and its role in cell death. Biochemical Journal. ,vol. 341, pp. 233- 249 ,(1999) , 10.1042/BJ3410233
Manon van Engeland, Luc J.W. Nieland, Frans C.S. Ramaekers, Bert Schutte, Chris P.M. Reutelingsperger, Annexin V‐Affinity assay: A review on an apoptosis detection system based on phosphatidylserine exposure Cytometry. ,vol. 31, pp. 1- 9 ,(1998) , 10.1002/(SICI)1097-0320(19980101)31:1<1::AID-CYTO1>3.0.CO;2-R
John D. Robertson, Sten Orrenius, Boris Zhivotovsky, Review: Nuclear Events in Apoptosis Journal of Structural Biology. ,vol. 129, pp. 346- 358 ,(2000) , 10.1006/JSBI.2000.4254
Vladimir Gogvadze, Sten Orrenius, Boris Zhivotovsky, Multiple pathways of cytochrome c release from mitochondria in apoptosis. Biochimica et Biophysica Acta. ,vol. 1757, pp. 639- 647 ,(2006) , 10.1016/J.BBABIO.2006.03.016
Nora M.A. Ponce, Carlos A. Pujol, Elsa B. Damonte, Marı&#x;a L. Flores, Carlos A. Stortz, Fucoidans from the brown seaweed Adenocystis utricularis: extraction methods, antiviral activity and structural studies Carbohydrate Research. ,vol. 338, pp. 153- 165 ,(2003) , 10.1016/S0008-6215(02)00403-2
Douglas R Green, Guido Kroemer, The Pathophysiology of Mitochondrial Cell Death Science. ,vol. 305, pp. 626- 629 ,(2004) , 10.1126/SCIENCE.1099320
Meilan Xue, Yinlin Ge, Jinyu Zhang, Qing Wang, Lin Hou, Yongchao Liu, Lingling Sun, Quan Li, Anticancer Properties and Mechanisms of Fucoidan on Mouse Breast Cancer In Vitro and In Vivo PLoS ONE. ,vol. 7, pp. e43483- ,(2012) , 10.1371/JOURNAL.PONE.0043483
Liping Ma, Cuili Qin, Mingchun Wang, Dan Gan, Lin Cao, Hong Ye, Xiaoxiong Zeng, Preparation, preliminary characterization and inhibitory effect on human colon cancer HT-29 cells of an acidic polysaccharide fraction from Stachys floridana Schuttl. ex Benth Food and Chemical Toxicology. ,vol. 60, pp. 269- 276 ,(2013) , 10.1016/J.FCT.2013.07.060