Dihydroxy-isosteviol-methyl-ester, an active biological component of Pulsatilla nigricans, reduces arsenic induced cellular dysfunction in testis of male mice.

摘要: Arsenic contamination has become a menacing health concern, warranting search for new drugs capable of ameliorating its toxicity. Extract Pulsatilla nigricans is occasionally used as traditional medicine including homeopathy to combat/alleviate toxicity-related symptoms known or unknown cause. Mice were intoxicated with sub-lethal dose sodium arsenite (20mg/kg b.w./day, determined through range-finding trial) and the effect on testicular toxicity after 30, 60, 90 days was examined. We observed an increased level reactive oxygen species, cellular damage in testes SA-intoxicated mice further analysed expressions apoptotic signal proteins mRNA like Bax, Bcl2 caspase3. Treatment EEPN showed significant inhibition/reversal arsenic-induced toxic testis reduced oxidative stress modulating proteins, thereby inhibiting progression events apoptosis cells sperm. Therefore, potentials therapeutic use arsenic- induced reproductive