Coronavirus and paramyxovirus in bats from Northwest Italy.

摘要: Bat-borne virus surveillance is necessary for determining inter-species transmission risks and important due to the wide-range of bat species which may harbour potential pathogens. This study aimed monitor coronaviruses (CoVs) paramyxoviruses (PMVs) in bats roosting northwest Italian regions. Our investigation was focused on CoVs PMVs their proven ability switch host zoonotic potential. Here we provide phylogenetic characterization highly conserved polymerase gene fragments. Family-wide PCR screenings were used test 302 belonging 19 different species. Thirty-eight animals from 12 locations confirmed as positive, with an overall detection rate 12.6% [95% CI: 9.3–16.8]. CoV RNA found 36 eight species, while PMV three Pipistrellus spp. Phylogenetic have been obtained 15 alpha- CoVs, 5 beta-CoVs PMVs; moreover one P. pipistrellus resulted co-infected both PMV. A divergent alpha-CoV clade Myotis nattereri SpA also described. The compact cluster R. ferrumequinum roosts expands current viral sequence database, specifically this Europe. To our knowledge first report Plecotus auritus M. oxygnathus, kuhlii. identified alpha new previously unsurveyed represents unique Italy. 23 genetic sequences presented will expand molecular bat-borne databases. Considering amount novel associated emergence infections humans last years, definition diversity within European needed. Performing studies a specific geographic area can awareness burden where roost close proximity spillover hosts, form basis appropriate control measures against threats public health optimal management habitats.