Transforming growth factor-beta 1 localization in normal and psoriatic epidermal keratinocytes in situ.

摘要: Transforming growth factor-beta 1 (TGF beta 1) is a potent inhibitor of epithelial cell proliferation and its effects on differentiation have been extensively characterized in cultured keratinocytes. We used two TGF 1-specific polyclonal antibodies (anti-LC anti-CC) to determine the presence peptide keratinocytes sections normal human skin situ both plaque nonplaque from individuals with psoriasis. In contrast phenotype expressed by epidermis, psoriatic exhibit hyperproliferative/regenerative phenotype. Anti-TGF staining was observed primarily epidermis. Anti-LC antibody stained nonproliferating, differentiated suprabasal intracellularly but did not stain plaques five seven patients. contrast, anti-CC extracellularly plaques, skin. Both anti-LC Thus, conformation or structure localization vary distinct phenotypes suggesting that potential modulator keratinocyte vivo. Selective association nonproliferating layers epidermis exclusion proliferating basal layer suggest it may be physiological regulator proliferation. addition, intracellular suggests constitutively synthesized epidermal