Comparison of three different techniques to diagnose Fasciola hepatica infection in experimentally and naturally infected sheep

摘要: The aim of this study was to compare three different techniques for the early diagnosis infection by Fasciola hepatica in experimentally and naturally infected sheep. experimental group consisted 7 sheep with 200 metacercariae; faecal samples were taken weekly until 12 week post-infection (wpi). Under natural conditions, 45 individual 2 pools faeces collected from flocks a history F. infection. results obtained coprological method compared commercial immunoassay two PCR assays samples. Faecal eggs detected 9 wpi On other hand, only 24 out positive flocks. By means sandwich-ELISA kit, first 4 57.1% percentage reached 100% 8 wpi. All animals method. Regarding PCR, specific 423 bp fragment mitochondrial DNA amplified 3 standard nested-PCR. Only 37 although diagnosed all In conclusion, sensitivity nested-PCR described our is higher than detection as well immunoassay. Moreover, no cross reactions gastrointestinal nematodes.