Colloid, adhesive and release properties of nanoparticular ternary complexes between cationic and anionic polysaccharides and basic proteins like bone morphogenetic protein BMP-2.

摘要: Herein we describe an interfacial local drug delivery system for bone morphogenetic protein 2 (BMP-2) based on coatings of polyelectrolyte complex (PEC) nanoparticles (NP). The application horizon is the functionalization substituting materials (BSM) used therapy systemic diseases. Nanoparticular ternary complexes cationic and anionic polysaccharides BMP-2 or two further model proteins, respectively, were prepared in dependence molar mixing ratio, pH value polysaccharide. As proteins chymotrypsin (CHY) papain (PAP) selected, which served as due to similar isoelectric points molecular weights. charged ethylenediamine modified cellulose (EDAC) trimethylammonium (PQ10) combined with sulphatesulfate (CS). Mixing diluted polysaccharide solutions according a slight either excess charge colloidal dispersions formed, cast onto germanium substrates by water evaporation. Dynamic light scattering (DLS) demonstrated, that these colloidally stable at least one week. Fourier Transform Infrared (FTIR) showed, loaded PEC NP irreversibly adhesive substrate contact HEPES buffer solely CHY, PAP released within long-term time scale. Advantageously, out three showed smallest initial burst slowest release kinetics around 25% content 14days. Released significant activity myoblast cells indicating ability regulate formation new bone. Therefore, are suggested novel promising tool BSM