Langerhans cells are more efficiently transduced than dermal dendritic cells by adenovirus vectors expressing either group C or group B fibre protein: implications for mucosal vaccines.

摘要: Vaccines against viruses need to target dendritic cells (DC) and stimulate mucosal immunity. Most vaccine studies have focussed on monocyte-derived or dermal DC (dDC) but recent evidence suggests that Langerhans (LC) may immunity more effectively. New chimeric adenovirus vectors expressing fibre protein from group B adenoviruses (rAd5/11), which utilise CD46 rather than the Coxsackie receptor (CAR), been developed as vaccines improve transduction overcome problems of pre-existing vector Transduction LC dDC by rAd5/11 standard rAd5 green fluorescent (GFP) showed both types were efficiently transduced rAd5. Although expression integrins αvβ3 αvβ5, recognise penton base mediate virus internalisation, was similar in dDC, expressed higher levels GFP. electroporation plasmid also resulted GFP LC, suggesting differences between two populations at a post-entry stage. with either did not induce maturation affect their ability T cells. These findings suggest strategies be worthy exploration.