Analysis of differential protein expression in Acidithiobacillus ferrooxidans grown under different energy resources respectively using SELDI-ProteinChip technologies.
作者： Zhiguo He , Hui Zhong , Yuehua Hu , Shengmu Xiao , Jin Xu
DOI: 10.1016/J.MIMET.2005.06.006
关键词:
摘要: Abstract Surface-enhanced laser desorption/ionization (SELDI)-time of flight is an affinity-based mass spectrometric method in which proteins interest are selectively absorbed to a chemically modified surface on chip, allows proteomic analysis with limited material requirements. This characteristic makes it valuable technique for microbiologists handling problematic samples, such as low cell number cultures. In this study, we explored differential-expressed proteome Acidithiobacillus ferrooxidans cultivated Fe 2+ and elemental sulfur separately by adopting the protein biochip SELDI approach. The lysates A. were applied onto Ciphergen ProteinChip WCX2, SAX2 IMAC-Cu arrays. Proteins bound chips analyzed Reader Model PBS II. A summary molecular masses differentially regulated found was obtained 28 expressed weight range 5.0 25 kDa.
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