Noradrenaline Stimulation Unbalances the Phosphoinositide Cycle in Rat Cerebral Cortical Slices
作者： Enrique Claro , John N. Fain , Fernando Picatoste
DOI: 10.1111/J.1471-4159.1993.TB03492.X
关键词:
摘要: Muscarinic cholinergic and alpha 1-adrenoceptor-mediated stimulation of phosphoinositide hydrolysis in rat cerebral cortex were compared by measuring carbachol- noradrenaline-induced accumulation various intermediates the cycle. Unlike carbachol, noradrenaline presence guanosine 5'-O-(3-thiotriphosphate) did not stimulate phospholipase C activity brain cortical membranes. In slices, efficacy to 3H-inositol phosphates [32P]phosphatidic acid was 2.5 threefold that carbachol. However, less effective than carbachol stimulating [3H]CDP-diacylglycerol resynthesis phosphatidylinositol. This due calcium inhibition CTP:phosphatidate cytidyltransferase or different lithium requirements for noradrenaline-stimulated [3H]CDP-diacylglycerol. The unbalance cycle, which most apparent at relatively high concentrations (2.5 mM) incubation buffer, qualitatively reproduced with ionomycin. use 1a-subtype-selective adrenoceptor antagonists WB4101 5-methylurapidil revealed a single 1a-like component mediating effects noradrenaline. Our results suggest primary mechanism activation 1 adrenoceptors involves an increase intracellular concentration.
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