Phosphoinositide-specific phospholipase C activation in brain cortical membranes
作者： Michael A. Wallace , Enrique Claro , Helen R. Carter , John N. Fain
DOI: 10.1016/0076-6879(91)97144-N
关键词:
摘要: Publisher Summary This chapter describes a recently developed assay for carbachol-activated, guanine nucleotide-dependent phospholipase C stimulation using crude membrane preparations from rat brain cortex. The procedure is generally applicable to most regions, and it has been successfully used with rabbit brain. results of this demonstrate that carbachol (PLC)-β seen only in the presence guanosine-5′-triphosphate (GTP) or nonhydrolyzable GTP analog such as GppNHp GTPγS. Other purine pyrimidine nucleotides do not substitute. responses mediated by GTPγS are effectively inhibited GDP GDPβS. Thus, apparently works through G protein activate PLC-β. proteins mediate inhibitory inputs into signaling system. Dopamine inhibits activation PLC-β cortical membranes pharmacological characterization reveals effect dopamine occurs D l -type receptor. A dual NaF found there PLC activity an inhibition PI kinase. Addition AICl 3 potentiates former but no influence on latter. therefore suggest controls kinase membranes.
sci-hub.se 参考文章(14)
C. Peter Downes, Robert H. Michell, The polyphosphoinositide phosphodiesterase of erythrocyte membranes Biochemical Journal. ,vol. 198, pp. 133- 140 ,(1981) , 10.1042/BJ1980133
E Claro, M A Wallace, H M Lee, J N Fain, Carbachol in the presence of guanosine 5'-O-(3-thiotriphosphate) stimulates the breakdown of exogenous phosphatidylinositol 4,5-bisphosphate, phosphatidylinositol 4-phosphate, and phosphatidylinositol by rat brain membranes Journal of Biological Chemistry. ,vol. 264, pp. 18288- 18295 ,(1989) , 10.1016/S0021-9258(18)51461-7
Hikaru HARAFUJI, Yasuo OGAWA, Re-Examination of the Apparent Binding Constant of Ethylene Glycol Bis(β-Aminoethyl Ether)-N,N,N′N′-Tetraacetic Acid with Calcium around Neutral pH Journal of Biochemistry. ,vol. 87, pp. 1305- 1312 ,(1980) , 10.1093/OXFORDJOURNALS.JBCHEM.A132868
E Claro, M A Wallace, J N Fain, Dual effect of fluoride on phosphoinositide metabolism in rat brain cortex. Stimulation of phospholipase C and inhibition of polyphosphoinositide synthesis. Biochemical Journal. ,vol. 268, pp. 733- 737 ,(1990) , 10.1042/BJ2680733
Helen R. Carter, Michael A. Wallace, John N. Fain, Purification and characterization of PLC-βm, a muscarinic cholinergic regulated phospholipase C from rabbit brain membrane Biochimica et Biophysica Acta. ,vol. 1054, pp. 119- 128 ,(1990) , 10.1016/0167-4889(90)90213-W
John N. Fain, Regulation of phosphoinositide-specific phospholipase C. Biochimica et Biophysica Acta. ,vol. 1053, pp. 81- 88 ,(1990) , 10.1016/0167-4889(90)90029-D
Helen R. Carter, Michael A. Wallace, John N. Fain, Activation of phospholipase C in rabbit brain membranes by carbachol in the presence of GTPγS; effects of biological detergents Biochimica et Biophysica Acta. ,vol. 1054, pp. 129- 135 ,(1990) , 10.1016/0167-4889(90)90214-X
Michael A. Wallace, Enrique Claro, A novel role for dopamine: inhibition of muscarinic cholinergic-stimulated phosphoinositide hydrolysis in rat brain cortical membranes. Neuroscience Letters. ,vol. 110, pp. 155- 161 ,(1990) , 10.1016/0304-3940(90)90804-I
S. Rhee, P. Suh, S. Ryu, S. Lee, Studies of inositol phospholipid-specific phospholipase C. Science. ,vol. 244, pp. 546- 550 ,(1989) , 10.1126/SCIENCE.2541501
D M Chuang, Neurotransmitter Receptors and Phosphoinositide Turnover Annual Review of Pharmacology and Toxicology. ,vol. 29, pp. 71- 110 ,(1989) , 10.1146/ANNUREV.PA.29.040189.000443