[3] Effects of microheterogeneity on sedimentation patterns of interacting proteins and the sedimentation behavior of systems involving two ligands

摘要: Publisher Summary The chapter describes various model interactions that broaden the theoretical base for biophysical investigations into architectural and regulatory roles played by protein association in vivo. A rather provocative result is microheterogeneity with respect to constant can give sedimentation patterns exhibiting three peaks, but other mechanisms also rise trimodal patterns. These include associations mediated two ligands, system, monomer ⇄ trimer nonamer, slow reaction rates. In regard conventional ultracentrifugal analysis, many of displayed herein bear a strong resemblance shown mixtures noninteracting proteins. Proof inherent heterogeneity depends upon isolation components. related vein, results self-association ligands could conceivably have import active enzyme solutions containing allosteric affectors, cofactors, or inhibitors, which promote as case carbamoylphosphate synthetase from E. coli .