Regulation of Cell Morphology in B Lymphocytes by IL-4: Evidence for Induced Cytoskeletal Changes

摘要: Lymphocyte activation is often accompanied by changes in cell morphology, for example, adhesion or motility. IL-4 a cytokine exerting many effects on B lymphocytes. In this study, we show that stimulation with LPS combination IL-4, but not alone, results pronounced dendritic morphology of cells. Using culture system which Abs directed to surface markers are immobilized the tissue plastic, find spreading can be mediated variety Abs, including anti-CD44, -CD23, -LFA-1, -VLA-4, -ICAM-1, and -Ig. cells stimulated anti-Ig plus anti-CD40 presence absence also induced spread, while IL-2, IL-5, IL-10 alone fail induce this. Spreading correlates induction tight aggregation. It sensitive cytochalasin B, indicating requirement intact actin cytoskeleton. CD44 selectively detected detergent-insoluble fraction lysates prepared from IL-4-stimulated cultures after Ab cross-linking CD44, suggesting membrane protein-cytoskeleton interaction. Interestingly, electron microscopy studies reveal microvilli-like structures blasts, influence an ultra-structural level. summary, our data ligation CD40 capable inducing dramatic morphologic murine cells, vitro strong adhesion.