Establishment and characterization of an ovarian cell line of the silkworm, Bombyx mori.

摘要: Abstract A cell line BmN-SWU1 was established from the ovarian tissues of 3-day-old fourth instar Bombyx mori larvae 21-872nlw variety by performing primary cultures in Grace's medium supplemented with 20% fetal bovine serum (FBS). The primarily consisted short spindle cells and round cells. frequency chromosome number 2 n  = 56 80.5%; therefore, considered to be a diploid line. population-doubling time (PDT) at 45th passage 57.7 h. This susceptible B. nuclear polyhedrovirus (BmNPV), median tissue culture infective dose (TCID 50 ) density 10 5 cells/ml 16.3 OBs/ml. transient expression efficiency green fluorescent protein (GFP) gene this 54.8%. We used select establish GFP transgenic