Three-Dimensional Cultures of Normal and Malignant Human Breast Epithelial Cells to Achieve in vivo-like Architecture and Function

摘要: Publisher Summary This chapter describes an assay in which primary or phenotypically normal human breast epithelial cells, cultured a laminin-rich basement membrane (lrBM), undergo three-dimensional reorganization to form structures that mimic vivo acinar culture. Primary luminal cells from reduction mammoplasty as well mammary cell lines have been successfully utilizing the 3D BM assay. The HMT3522 progression series consists of immortal originally isolated fibrocystic tissue and includes S1 their tumorigenic derivative T4-2 were selected for ability grow absence EGF. Successful growth these requires special attention media, confluency, trypsinization, feeding regime order maintain healthy capable undergoing differentiation. Single embedded into lrBM will several rounds division, withdraw cycle between 6 8 days depending on type, organize polarized structures, acini-like including central lumen.