Modeling breast acini in tissue culture for detection of malignant phenotype reversion to non-malignant phenotype.

摘要: Backgrounds: Evidence is accumulating to support disruption of tissue architecture as a powerful event in tumor formation. For the past four decades, intensive cancer research with premise “cancer cell based-disease” focused on finding oncogenes or suppressor genes. However, role was neglected. Three dimensional (3D) cultures which can recapitulate major aspects microenvironment are appropriate models for exploring cancer. first time Iran, we have launched Matrigel based non-malignant, tumorigenic and reverted breast 3D cultures. Methods: Non-tumorigenic MCF-10A MCF-7 lines were cultured plastic Matrigel. normal phenotype via AIIB2 LY 294002 inhibitors against β1 integrin class I phosphatidylinositol 3-kinase, respectively. Results: acini distinguishably different from formed organized hollow spherical structures stark contrast disorganized cluster cells. allowed visual monitoring cells treated inhibitors. After treatment cells, observed reversion toward normal, comparable acini. Conclusion: The culture provides allows malignant non-malignant demonstrate near physiological behavior this distinguish nonmalignant also spheres. Iran. Biomed. J. 13 (4): 191-198, 2009