Influence of irradiance on chlorophyll synthesis in Picea abies calli cultures

作者: R. Balazova , A. Blehova , V. Demko , K. Breznenova , J. Hudak

DOI: 10.1007/S10535-011-0027-5

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摘要: Dark-grown seedlings of Picea abies (L) Karst. are able to accumulate the highest amounts chlorophyll (Chl) and its precursor protochlorophyllide (Pchlide) in all Pinaceae, but calli derived from 14-d-old green cotyledons P. completely white during cultivation dark. Pchlide reduction is catalysed dark by light-independent oxidoreductase (DPOR). This enzyme complex consists three protein subunits ChlL, ChlN ChlB, encoded plastid genes chlL, chlN chlB. Using semiquantitative RT-PCR, we observed very low expression chlLNB dark-grown calli. It seems, that thus Chl accumulation could be modulated light cultures. hypothesis supported fact, contents glutamyl-tRNA reductase Flu-like protein, which probably affected biosynthetic pathway at step 5-aminolevulinic acid formation. ChlB subunit was not detected Our results indicated limited ability synthesize callus

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