Molecular analysis of the rlsA gene regulating levan production by the fireblight pathogen Erwinia amylovora

摘要: Abstract Natural Erwinia amylovora can display a reduced level of levan synthesis. A gene ( rlsA ) responsible for the suppression low levansucrase synthesis by E. was cloned complementation natural levan-deficient strains lacking expression. The region sequenced, and an open reading frame 266 amino acids found. Sequence analysis revealed that RlsA resembles transcriptional activator may be member LysR family. Overexpression from high copy number plasmid led to two-fold increase production, but had no influence on amylovoran mutant created site-directed mutagenesis wild type strain Eal/79 same phenotype as strains. deficient (with exception PD494) could amplified PCR, yielding DNA fragments absent in with large deletion affecting hrp . match nucleotide sequence database its identity incomplete located downstream two recently characterised dsp genes.