Modulation of Calcium Current in Arteriolar Smooth Muscle by αvβ3 and α5β1 Integrin Ligands

摘要: Vasoactive effects of soluble matrix proteins and integrin-binding peptides on arterioles are mediated by αvβ3 α5β1 integrins. To examine the underlying mechanisms, we measured L-type Ca2+ channel current in arteriolar smooth muscle cells response to integrin ligands. Whole-cell, inward Ba2+ currents were inhibited after application cyclic RGD peptide, vitronectin (VN), fibronectin (FN), either two anti–β3 antibodies, or monovalent β3 antibody. With VN antibody coated onto microbeads presented as an insoluble ligand, was also inhibited. In contrast, beads with FN α5 produced significant enhancement bead attachment. Soluble had no effect but blocked increase evoked FN-coated enhanced when applied combination appropriate IgG. The data suggest that integrins differentially linked through intracellular signaling pathways thereby alter control influx vascular muscle. This would account for vasoactive ligands provide a potential mechanism wound recognition during tissue injury.