CXCL5, CXCL8, and CXCL10 regulation by bacteria and mechanical forces in periodontium.

摘要: Abstract Objective The aim of the present study was to evaluate expressions CXCL5, CXCL8, and CXCL10 in periodontal cells tissues response microbial signals and/or biomechanical forces. Methods Human gingival biopsies from inflamed healthy sites were used examine chemokine protein levels by real-time PCR immunohistochemistry. chemokines also investigated rats submitted experimental periodontitis tooth movement. Furthermore, determined human fibroblasts stimulated periodontopathogen Fusobacterium nucleatum constant tensile forces (CTS) ELISA. Additionally, gene evaluated exposed F. CTS presence absence a MAPK inhibitor PCR. Results Increased observed rat gingiva inflammation as compared with health. caused significant (p  Conclusions Our data provide original evidence that strain further increases stimulatory actions bacteria on these chemokines. Therefore, loading combination infection may lead stronger recruitment immunoinflammatory periodontium, which might result an aggravation destruction.