Genomic Analysis of Serogroup Y Neisseria meningitidis Isolates Reveals Extensive Similarities Between Carriage-Associated and Disease-Associated Organisms

摘要: Background. Neisseria meningitidis is a frequent colonizer of the human nasopharynx with asymptomatic carriage providing reservoir for invasive, disease-causing strains. Serogroup Y (MenY) strains are major cause meningococcal disease. High resolution genetic analyses and disease isolates can establish epidemiological relationships identify potential virulence factors. Methods. Whole genome sequence data were obtained from UK MenY 1997-2010 (n=99). Sequences compared to those invasive 2010 2011 (n=73) using gene-by-gene approach. Results. Comparisons across 1,605 core genes resolved 91% into one eight clusters containing closely related isolates. Six contained carried meningococci isolated in 1997-2001 suggesting temporal stability. One cluster isolates, predominately sharing designation Y: P1.5-1,10-1: F4-1: ST-1655 (cc23), was sub-cluster 86% second 90% These subclusters defined by specific allelic differences five encoding glycerate kinase (glxK), valine-pyruvate transaminase (avtA), superoxide dismutase (sodB) two hypothetical proteins. Conclusions. detected long-term stability temporally-overlapping populations clones but also evidence disease-associated clone.