Mitotic regulator protein RCC1 is complexed with a nuclear ras-related polypeptide.

摘要: Abstract We previously reported the purification of a complex two proteins from human chromatin, consisting 47-kDa component called RCC1, which is negative regulator mitosis, and 25-kDa protein. Here we show that protein has ras-related sequence. It binds guanine nucleotides, excess Mg2+ GDP or GTP dissociate complex. Immunofluorescence studies biochemical properties indicate this polypeptide, in contrast to most members Ras family, present nucleoplasm as soluble monomer, 25-fold over complexed form. We designate polypeptide Ran, for nuclear