Cytotoxic and cytoprotective activities of curcumin. Effects on paracetamol-induced cytotoxicity, lipid peroxidation and glutathione depletion in rat hepatocytes.

摘要: The cytoprotective effect of curcumin, a natural constituent Curcuma longa, on the cytotoxicity paracetamol in rat hepatocytes was studied. Paracetamol selected as model-toxin, since it is known to be bioactivated by 3-methylcholanthrene inducible cytochromes P450 presumably N-acetyl-p-benzoquinone imine (NAPQI), reactive metabolite which upon overdosage causes protein- and non-protein thiol-depletion, lipid peroxidation measured LDH-leakage. At low concentrations curcumin found protect significantly against paracetamol-induced peroxidation, without protection LDH-leakage GSH-depletion. 100 times higher concentration observed protective accompanied with tendency increase cellular GSH-depletion No time-dependency curcumin-induced effects: treatment 1 hr before, concomitantly or after addition cells had similar effects. In contrast what expected basis previous vivo experiments, at itself slightly cytotoxic. Curcumin-induced significant depletion GSH. It has been concluded that cytotoxic activities may explained strong anti-oxidant capacity capability conjugate Furthermore, not playing causal role cell-death induced curcumin.