Cloning and analysis of an Escherichia coli operon containing the rpmF gene for ribosomal protein L32 and the gene for a 30-kilodalton protein.

摘要: The chromosomal DNA fragments of Escherichia coli K-12 were cloned into a mini-F cosmid, pRE435, after partial digestion with restriction endonuclease Sau3AI. clones first screened for PyrC+ and then other genes, including rpmF encoding ribosomal protein L32 that had been mapped near pyrC (I. Janda, M. Kitakawa, K. Isono, Mol. Gen. Genet. 201:443-436, 1985). Thus, we obtained total five rpmF-containing clones. gene was located on the segment in one (pAY2-5) by insertional mutagenesis transposon gamma delta, followed analysis products maxicell method. Hybridization clone pAY2-5 ordered bank (Y. Kohara, Akiyama, Cell 50:495-508, 1987) indicates gap at 1,510-kilobase coordinates physical map E. can be bridged this clone. nucleotide sequence region containing accordingly established. In addition, RNA transcripts from analyzed, transcriptional initiation sites determined. results suggest forms an operon termed g30k which codes 30-kilodalton unknown function. At least four found to code L32.