Selective targeting of somatostatin receptor 3 to neuronal cilia

摘要: Abstract Recently, five members of the somatostatin receptor family have been cloned. However, little is known about their cellular and subcellular localization in central nervous system. Using specific anti-peptide antisera, we observed 3-like immunoreactivity many brain regions, including cerebral cortex, hippocampus, hypothalamus, amygdala cerebellum. In all these regions (except for cerebellar cortex), was selectively targeted to 4–8-μm-long rod-shaped profiles which did not co-localize with axonal or dendritic markers. One immunoreactive profile always associated one neuronal cell body. This staining pattern resistant colchicine treatment showed a closely overlapping distribution 3 messenger RNA, suggesting that protein transported over long distances. Electron microscopic analysis revealed localized plasma membrane cilia extended into an intercellular pocket 9+0 filament basal body proximal segments. Thus, demonstrates unique example G-protein-coupled “classical” pre- postsynaptic sites, but cilia. The presence on suggests presumably non-motile may merely represent developmental remnants, rather function as chemical sensors immediate milieu.