Immunocytochemistry and in situ hybridization of catecholamine-synthesizing enzymes and the related neurotransmitters

摘要: Publisher Summary This chapter focuses on immunocytochemistry and in situ hybridization of catecholamine (CA)-synthesizing enzymes the related neurotransmitters. It describes methods neurotransmitters or neuropeptides for CA-synthesizing enzymes, mRNAs results obtained by these methods. presents a comparison various histochemical methods—the histofluorescence technique monoamines, immunohistochemistry using antisera to immunoenzyme histochemistry antibodies against monoamine-synthesizing mRNA enzymes. The discusses distribution central monoamine systems mouse, polyclonal serotonin. principles immunocytochemical including immunofluorescence, labeled antibody, peroxidase-antiperoxidase, protein-A-gold, avidin biotin complex procedures. also phylogenetic ontogenetic studies CA immunocytochemistry.