The quantitative bioassay of nerve growth factor: use of frozen ‘primed’ PC12 pheochromocytoma cells

摘要: A quantitative bioassay for nerve growth factor (NGF) has been previously described which employs PC13 rat pheochromocytoma cells. The basis of this assay is that rapid neurite regeneration by PC12 cells pretreated ('primed') with NGF at least a week quantitatively related to the amount present in culture medium. experiments demonstrate NGF-pretreated retain their priming over year when appropriately frozen and stored such can be employed NGF. major advantage large numbers primed time (in suspension culture) aliquots until future use. newly essentially as reagents avoids need have freshly cultures on hand.