[16] Derivation of monoclonal antibody to nerve growth factor

摘要: Publisher Summary Nerve growth factor (NGF) is essential for the differentiation of neural crest during development and required maintenance sympathetic sensory neurons in culture vivo. Although NGF not a mitogen, it shares many features with epidermal factor, insulin, other protein hormones, such as interaction plasma membrane receptor, internalization, pleiotropic signals to target neuron. The procedures described chapter demonstrate that problems inherent production monoclonal antibodies immunological studies mouse can be essentially overcome several effective ways. results from species specificity analysis variety effects 12 on bioassay suggest there are at least 3 epitopes 13,000-DaβNGF, one or more which shared species. Binding present consistent thermodynamic picture. A sensitive highly specific immunoassay utilizing these has yet been reported, but could readily developed radioimmunoassay (RIA) enzyme linked immunosorbent assay (ELISA) techniques already further refined by incorporation two-site RIA polyclonal antibodies. These reagents should also useful affinity purification immunocytochemistry. More insight into biochemistry molecule, its responsive neurons, biological provided