Involvement of leukemia inhibitory factor and nerve growth factor in the development of dorsal root ganglion neurons

摘要: Leukemia inhibitory factor (LIF) was recently shown to stimulate the generation of sensory neurons from murine neural crest in vitro. Here, we examine respective activities LIF and nerve growth (NGF) throughout embryonic development dorsal root ganglia (DRG) crest. In cultures day 12 (E12) DRG, which contain neuron precursor cells, a combination both NGF are required for differentiation mature their neurofilament negative (NF-) precursors. The primary step NF- cell NF+ immature is promoted by LIF, whereas survival further maturation newly differentiated depends on NGF. isolated at time target innervation (E14 E15 DRG), majority dependent However, acts as agent discrete population non-responsive neurons. From E16, number maintained increases > 90% birth. Consistent with vitro observations, mRNA could be detected early developmental stages (E12-E13), within spinal column DRG well limbs and, later (after E15), areas (skin, limbs, feet gut). This supports idea that NGF, may regulate vivo.