Focal adhesion kinase regulates cell-cell contact formation in epithelial cells via modulation of Rho.

摘要: Focal Adhesion Kinase (FAK) is a non-receptor tyrosine kinase that plays key role in cellular processes such as cell adhesion, migration, proliferation and survival. Recent studies have also implicated FAK the regulation of cell-cell adhesion. Here, evidence presented showing siRNA-mediated suppression levels NBT-II cells expression dominant negative mutants caused loss epithelial morphology inhibited formation adhesions. Rac Rho been adhesions can be regulated by signaling. Expression active or disrupted contacts, thus promoting phenotype similar to FAK-depleted cells. The intercellular contacts prevented upon mutant, but not mutant. Inhibition decreased phosphorylation p190RhoGAP elevated level GTP-bound Rho. This suggests regulates contact