Identification, cellular localization, isolation, and characterization of human Clara cell-specific 10 KD protein.:

摘要: Human lung lavage proteins were fractionated by centrifugation and molecular sieving. An antiserum to the post-albumin fraction of soluble reacted with a 10 KD protein this was isolated conventional chromatography. The protein, which has pI 4.8, consists two 5 polypeptides is rich in glutamic acid, leucine, serine, aspartic acid amino acids. does not bind concanavalin A, pancreatic elastase, leukocyte or trypsin, lacks anti-protease activity. It constitutes about 0.15% lavage. Antibodies specifically exclusively stain Clara cells human, dog, rat. Staining granules prominent distal bronchioles; however, non-ciliated respiratory bronchioles did for protein. This appears fetal lungs at 21 weeks gestation, present 10% primary pulmonary adenocarcinomas. As specific m...