Vascular endothelial growth factor (VEGF) regulation by hypoxia inducible factor-1 alpha (HIF1A) starts and peaks during endometrial breakdown, not repair, in a mouse menstrual-like model

摘要: Study question How is vascular endothelial growth factor (VEGF) expression regulated by hypoxia inducible 1 alpha (HIF1A) during menstruation? Summary answer After progesterone (P4) withdrawal, HIF1A was activated and it directly up-regulated VEGF mRNA this regulation the highest endometrium breakdown in mouse menstrual-like model. What known already VEGF, an important angiogenic factor, to be essential for endometrial repair, particularly angiogenesis re-epithelialization. However, its upstream has not been fully clarified. HIF1 first transcription response closely associated with angiogenesis; also regulator of mRNA. design, size, duration We investigated changes after P4 withdrawal inhibition. The total number mice used 62. treatment model 8 days. Participants/materials, setting, methods human decidual stromal cells were established mimic menstruation. Protein expressions immunohistochemistry, Western blot quantitative PCR. direct interaction between Vegf promoter chromatin immunoprecipitation. inhibition vivo vitro achieved administration inhibitor siRNA knockdown, respectively. Main results role chance translocated nucleus from 16 h while at 12 h. bound breakdown, which peaked suppressed protein decidualized cells. Inhibition breakdown. Limitations, reasons caution Although confirmed cells, functional further determined. Wider implications findings Here, we report that complicate plays a key funding/competing interests National Nature Science Foundation China (No. 30901608), Basic Research Program (2010CB530403) Technology Support 2012BAI32B05). authors have no conflicts interest disclose. Trial registration This study clinical trial.