Mild stimulation improves neuronal survival in an in vitro model of the ischemic penumbra.

摘要: OBJECTIVE: In the core of a brain infarct, characterized by severely reduced blood supply, loss neuronal function is rapidly followed death. peripheral areas penumbra, damage initially reversible, and activity typically due to ischemia-induced synaptic failure. There limited understanding factors governing recovery or transition irreversible damage. Neuronal has been shown be crucial for survival. Consequently, hypoxia induced inactivity may contribute cell death, activation penumbral neurons possibly improves Adversely, increases ATP demand, balance should found between available energy sufficient activity. APPROACH: We monitored viability in an vitro model consisting (rat) networks on micro electrode arrays (MEAs) under controlled hypoxic conditions. tested effects optogenetic electrical during hypoxia. MAIN RESULTS: Mild stimulation yielded significantly better immediately after re-oxygenation, compared with no stimulation, 60%-70% higher survival rate 5 d. Stronger was not associated than suggesting that beneficial depend delicate energy. SIGNIFICANCE: show mild hypoxia/ischemia penumbra. This finding opposes current common belief suppression cornerstone neuroprotection cerebral ischemia, open new possibilities treatment secondary stroke.