Homeostatically regulated synchronized oscillations induced by short-term tetrodotoxin treatment in cultured neuronal network.

摘要: Homeostatic plasticity plays a critical role in the stability of neuronal activities. Here, with high-density hippocampal networks cultured on multi-electrode arrays (MEAs), transformation spontaneous firing patterns induced by 1microM tetrodotoxin was clarified. Once washed out after 4-h treatment, activities rose significantly spike rate increasing approximately three times, and synchronized burst oscillations appeared throughout network, cross-correlation coefficient between active sites rising from 0.06+/-0.03 to 0.27+/-0.05. The long-term recording showed that lasted for more than 4h before network recovered. These results suggest short-term treatment may induce homeostatically enhanced excitability, should be an indicator homeostatic network. Furthermore, non-invasive MEAs as novel sensing system is identified appropriate pharmacological investigations at level.