Porcine circovirus type 2 protective epitope densely carried by chimeric papaya ringspot virus-like particles expressed in Escherichia coli as a cost-effective vaccine manufacture alternative.

摘要: Porcine circovirus type 2 (PCV2) still represents a major problem to the swine industry worldwide, causing high mortality rates in infected animals. Virus-like particles (VLPs) have gained attention for vaccine development, serving both as scaffolds epitope expression and immune response enhancers. The commercial subunit vaccines against PCV2 consist of VLPs formed by self-assembly capsid protein (CP) expressed baculovirus vector system. In this work, protective was inserted into three different regions papaya ringspot virus (PRSV) CP, namely, N- C-termini predicted antigenic region located near N-terminus. Wild-type chimeric CPs were modeled silico, Escherichia coli, purified, visualized transmission electron microscopy. This is first report that shows formation using PRSV epitope-presentation scaffold. Moreover, it found localization strongly influences VLP length. Also, estimated yields at small-scale level ranged between 65 80 mg/L culture medium. Finally, induced levels immunoglobulin G immunized BALB/c mice, suggesting these can be used immunoprophylaxis PCV2.