Method for measuring analyte transport across cell membranes using X-ray fluorescence

作者: Eva R. Birnbaum , Lori J. Peterson , Jennifer A. Berger , Rebecca L. Miller , Benjamin P. Warner

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摘要: The present invention includes a method and apparatus for measuring the transport of an analyte through cell membrane. One or more cells expressing plurality ion channels are provided. These loaded with so that contain at least 10 picograms within volume defined by area x-ray excitation beam depth five times 1/e attenuation one characteristic signal as attenuated water. unloaded is removed amount in measured fluorescence.

参考文章(21)
Kohji Nakazawa, Hiroshi Mizumoto, Mitsuru Kaneko, Hiroyuki Ijima, Tomonobu Gion, Mitsuo Shimada, Ken Shirabe, Kenji Takenaka, Keizo Sugimachi, Kazumori Funatsu, Formation of porcine hepatocyte spherical multicellular aggregates (spheroids) and analysis of drug metabolic functions. Cytotechnology. ,vol. 31, pp. 61- 68 ,(1999) , 10.1023/A:1008040726236
George J. Havrilla, Benjamin P. Warner, Grace Mann, Method for detecting binding events using micro-X-ray fluorescence spectrometry ,(2001)
Cris L. Lewis, Cyndi A. Wells, George J. Havrilla, Thomasin C. Miller, Cynthia A. Mahan, Benjamin P. Warner, Flow method and apparatus for screening chemicals using micro x-ray fluorescence ,(2003)
L. Finney, S. Mandava, L. Ursos, W. Zhang, D. Rodi, S. Vogt, D. Legnini, J. Maser, F. Ikpatt, O. I. Olopade, D. Glesne, X-ray fluorescence microscopy reveals large-scale relocalization and extracellular translocation of cellular copper during angiogenesis. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 104, pp. 2247- 2252 ,(2007) , 10.1073/PNAS.0607238104
Lawrence A. Crum, David Tirrell, Pierre D. Mourad, Tyrone M. Porter, Patrick Stayton, Oliver W. Press, Chantal Lackey Reed, Niren Murthy, Allan S. Hoffman, Enhanced transport using membrane disruptive agents ,(1999)
Sarah D. Oeltjen, Stephen G. Kieffer-Higgins, Ted Minior, Bruce L. Carvalho, John P. Willis, Alec Mian, Laura L. Chapman, Gene A. Davis, Gregory Kellogg, Mikayla Kob, Shari Ommert, Devices and methods for using centripetal acceleration to drive fluid movement in a microfluidics system ,(1998)
George J. Havrilla, Thomasin C. Miller, Benjamin P. Warner, Cyndi A. Wells, Method and apparatus for detecting chemical binding ,(2004)
Andrew T. Koppisch, Jennifer A. Berger, Sharon M. Baldwin, Michael N. Harris, Jeffrey J. Stewart, Anthony K. Burrell, Benjamin P. Warner, Mark T. Mccleskey, Eva R. Birnbaum, Advanced drug development and manufacturing ,(2007)