Viruses budding from either the apical or the basolateral plasma membrane domain of MDCK cells have unique phospholipid compositions.

摘要: Influenza virus and vesicular stomatitis (VSV) obtain their lipid envelope by budding through the plasma membrane of infected cells. When monolayers Madin-Darby canine kidney (MDCK) cells, a polarized epithelial cell line, are with fowl plague (FPV), an avian influenza virus, or VSV, new FPV buds apical whereas VSV progeny is formed basolateral membrane. were isolated from MDCK host cells prelabeled [32P]orthophosphate phospholipid compositions compared. Infection was carried out at 31 degrees C to delay cytopathic effects infection, which lead depolarization surface. 32P-labeled culture medium, released below monolayer scraping dish 8 h after infection. At this time little found in indicating that still polarized. The composition two viruses distinctly different. enriched phosphatidylethanolamine phosphatidylserine phosphatidylcholine, sphingomyelin, phosphatidylinositol. trypsinized infection replated, non-infected control attached reform confluent within 4 h, remained suspension. could be suspension under these conditions very similar. We conclude lipids same way different reflect differences domains.