AN ALDOSTERONE-RELATED SYSTEM IN THE VENTROLATERAL MEDULLA OBLONGATA OF SPONTANEOUSLY HYPERTENSIVE AND WISTAR-KYOTO RATS

摘要: SUMMARY 1 The actions of aldosterone include mediation vasoconstriction, vascular fibrosis, endothelial dysfunction and sodium retention. These can contribute to hypertension. Recent studies implicate an abnormal hormonal system in the brain However, study central is still its infancy, as exact location abundance components are uncertain. 2 We aimed detect cascade regions ventrolateral medulla oblongata (VLM)-containing neurons that regulate blood pressure see whether there quantitative differences these between spontaneously hypertensive rat (SHR) normotensive Wistar-Kyoto (WKY) models. Tissues from four brainstem, namely, rostral caudal (RVLM CVLM, respectively), pressor area area, were examined. We measured mRNA expression synthase, mineralocorticoid receptor (MR1), 12-lipoxygenase (12-LO), serum- glucocorticoid- inducible kinase K-ras male rats. Gene levels using real-time reverse transcription–polymerase chain reaction. 3 We detected all VLM. The not significantly different any regions. Expression MR1 was lower RVLM SHR (n = 5) compared with WKY rats (n = 5; t = 4.590; P = 0.002) 12-LO CVLM (n = 6) (n = 7; P = 0.04). Thus, we have shown for first time present Our results suggest may be a differential gene profile brainstem genetic