Aldosterone is synthesized in and activates bulbospinal neurons through mineralocorticoid receptors and ENaCs in the RVLM

摘要: The effects of aldosterone and mineralocorticoid receptor (MR) blockers on presympathetic neurons in the rostral ventrolateral medulla (RVLM) are well studied. To directly investigate whether aldosterone, eplerenone (an MR blocker), FAD286 synthase inhibitor) benzamil epithelial sodium channel (ENaC) blocker) affect RVLM neurons, we examined changes membrane potentials (MPs) bulbospinal using whole-cell patch-clamp technique during superfusion with these drugs to brainstem–spinal cord preparations. Aldosterone (0.1 μmol/l) depolarized neurons. In contrast, (1 μmol/l) hyperpolarized them. evaluate existence (10 μmol/l) was performed, became FAD superfusion. These data suggest that MRs exist is synthesized brainstem. Benzamil clarify eplerenone, acted a low-Ca2+, high-Mg2+ solution used block synaptic input above-mentioned were added low-Ca2+ During superfusion, depolarized, they or Importantly, when superfused after solution, MPs did not depolarize. results present RVLM. themselves possess ENaCs, ENaCs underlying mechanism by which activates