Three types of putative presympathetic neurons in the rostral ventrolateral medulla studied with rat brainstem-spinal cord preparation.

摘要: Abstract To study the electrophysiological properties of presympathetic neurons in rostral ventrolateral medulla (RVLM), intracellular recordings were performed by whole-cell patch-clamp technique. We utilized neonatal rat brainstem–spinal cord preparation, which sympathetic neuronal network is thought to be preserved, unlike slice preparation. In response stimulation ipsilateral Th2 spinal segment including intermediolateral cell column (IML), 33 151 non-respiratory RVLM showed antidromic action potentials with a constant latency 45 ms, and can considered as neurons. classified characterized into three types: ‘regularly firing (n=7)’, ramp depolarization frequent (4.2±0.9 spikes/s) rare excitatory postsynaptic (EPSPs); ‘irregularly (n=21)’, exhibited many EPSPs that modulated rate; ‘silent-type (n=5)’, discharged only during current-induced depolarization. Lucifer-Yellow staining irregularly significantly larger had more dendrites than regularly All retained their discharges low-Ca2+–high-Mg2+ superfusion blocks synaptic input, whereas 11 16 abolished, suggesting independently input. Seven 31 hyperpolarized vagal afferent nerves. summary, types technique connection was preserved from through RVLM. Since demonstrated all types, constitute part network.