Gold nanoparticles conjugated to bimetallic manganese(II) and iron(II) Prussian Blue analogues for aptamer-based impedimetric determination of the human epidermal growth factor receptor-2 and living MCF-7 cells

摘要: An aptamer-based assay is described for the determination of trace levels cancer marker human epidermal growth factor receptor-2 (HER2) and living MCF-7 cells. The method based on use a bimetallic MnFe Prussian blue analogue coupled to gold nanoparticles (MnFePBA@AuNP). Compared pristine PBA nanocubes, series MnFePBA@AuNP exhibits core-shell spherical nanostructure, shell thickness decreases from 99.9 nm down 49.3 nm increasing fraction AuNPs. composite was placed electrode incubated with aptamer solution through electrostatic interaction. Then modified employed detect HER2 cells using [Fe(CN)6]3−/4− as redox probe displays good responses both them. Electrochemical impedance spectroscopy data show that signal variation between each step during whole procedure detection can be embodied resistance value change surface. has very low limit (0.247 pg∙mL−1) works in 0.001–1.0 ng∙mL−1 concentration range. It also used sense cells, this results an within 500–5 × 104 cell∙mL−1 cell range 36 limit. Furthermore, selective, acceptably reproducible, stable, well feasible serum.