Quantification of EGFR and EGFR-overexpressed cancer cells based on carbon dots@bimetallic CuCo Prussian blue analogue

摘要: A new bimetallic CuCo Prussian blue analogue (CuCo PBA) loaded with carbon dots (CDs) was prepared (represented by CD@CuCoPBA) and developed as a scaffold for anchoring the epidermal growth factor receptor (EGFR) aptamer to detect EGFR living EGFR-overexpressed cancer cells. The basic characterizations revealed PBA exhibited nanocube shape still remained its nanostructure physical/chemical properties after coupling large amounts of CDs. As compared pristine PBA, CD@CuCoPBA displayed good electrochemical activity, strong binding interaction toward aptamer, high stability aptamer-EGFR G-quadruplex in aqueous solution. such, results impedance spectroscopy measurements indicated that CD@CuCoPBA-based aptasensor an ultra-low detection limit (0.42 fg mL−1) MCF-7 cells (80 cell per mL), well selectivity, reproducibility, stability, repeatability, acceptable applicability. Consequently, constructed can be extended promising universal method early diagnosis cancers.