Determination of the Optimal Concentration of Several Selective Drugs Useful for Generating Multi-Transgenic Porcine Embryonic Fibroblasts
作者： M Sato , M Ohtsuka , H Miura , K Miyoshi , S Watanabe
DOI: 10.1111/J.1439-0531.2011.01964.X
关键词:
摘要: Contents Porcine embryonic fibroblasts (PEFs) are widely used as donor cells for somatic cell nuclear transfer (SCNT) in pigs. Transfection of PEFs with exogenous DNA is essential producing genetically modified (GM; transgenic or knockout) pigs via SCNT. In this case, selectable markers strictly required selecting and enriching stably transfected cells. The most frequently selective drug purpose a neomycin analogue (G418 ⁄ geneticin); neo has been marker gene the genomic manipulation However, little known about optimal concentrations other selection drugs. This often hampers functional analysis porcine genome development individual GM study explores drugs, than neomycin, that can be PEFs. were incubated media containing different drugs up to 10 days, determine fatal following found use PEFs: G418 geneticin, 400 lg ml; blasticidin S, 8 hygromycin B, 40 puromycin, 2 zeocin, 800 ml. Repeated transfections plasmids carrying resulted generation multidrug-resistant swine transfectants. Furthermore, these independent. present information will useful production SCNTmediated piglets express multiple transgenes.
sci-hub.se 参考文章(38)
Kaori KOBAYASHI, Takashi KAMAKURA, Teruo TANAKA, Isamu YAMAGUCHI, Toyoshige ENDO, Nucleotide sequence of the bsr gene and N-terminal amino acid sequence of blasticidin S deaminase from blasticidin S resistant Escherichia coli TK121. Agricultural and biological chemistry. ,vol. 55, pp. 3155- 3157 ,(1991) , 10.1271/BBB1961.55.3155
Victorine Douin, Stephanie Bornes, Laurent Creancier, Philippe Rochaix, Gilles Favre, Anne-Catherine Prats, Bettina Couderc, Use and comparison of different internal ribosomal entry sites (IRES) in tricistronic retroviral vectors. BMC Biotechnology. ,vol. 4, pp. 16- 16 ,(2004) , 10.1186/1472-6750-4-16
I. Wilmut, N. Beaujean, P. A. de Sousa, A. Dinnyes, T. J. King, L. A. Paterson, D. N. Wells, L. E. Young, Somatic cell nuclear transfer Nature. ,vol. 419, pp. 583- 587 ,(2002) , 10.1038/NATURE01079
Christopher S. Rogers, Yanhong Hao, Tatiana Rokhlina, Melissa Samuel, David A. Stoltz, Yuhong Li, Elena Petroff, Daniel W. Vermeer, Amanda C. Kabel, Ziying Yan, Lee Spate, David Wax, Clifton N. Murphy, August Rieke, Kristin Whitworth, Michael L. Linville, Scott W. Korte, John F. Engelhardt, Michael J. Welsh, Randall S. Prather, Production of CFTR-null and CFTR-ΔF508 heterozygous pigs by adeno-associated virus–mediated gene targeting and somatic cell nuclear transfer Journal of Clinical Investigation. ,vol. 118, pp. 1571- 1577 ,(2008) , 10.1172/JCI34773
Yoichi Takahagi, Tatsuya Fujimura, Shuji Miyagawa, Hiroshi Nagashima, Tamotsu Shigehisa, Ryota Shirakura, Hiroshi Murakami, Production of α1,3-galactosyltransferase gene knockout pigs expressing both human decay-accelerating factor and N-acetylglucosaminyltransferase III Molecular Reproduction and Development. ,vol. 71, pp. 331- 338 ,(2005) , 10.1002/MRD.20305
M. Sato, R. Iwase, K. Kasai, N. Tada, Direct injection of foreign DNA into mouse testis as a possible alternative of sperm‐mediated gene transfer Animal Biotechnology. ,vol. 5, pp. 19- 31 ,(1994) , 10.1080/10495399409525801
F. Schwartz, N. Maeda, O. Smithies, R. Hickey, W. Edelmann, A. Skoultchi, R. Kucherlapati, A dominant positive and negative selectable gene for use in mammalian cells Proceedings of the National Academy of Sciences of the United States of America. ,vol. 88, pp. 10416- 10420 ,(1991) , 10.1073/PNAS.88.23.10416
Liangxue Lai, Jing X Kang, Rongfeng Li, Jingdong Wang, William T Witt, Hwan Yul Yong, Yanhong Hao, David M Wax, Clifton N Murphy, August Rieke, Melissa Samuel, Michael L Linville, Scott W Korte, Rhobert W Evans, Thomas E Starzl, Randall S Prather, Yifan Dai, Generation of cloned transgenic pigs rich in omega-3 fatty acids. Nature Biotechnology. ,vol. 24, pp. 435- 436 ,(2006) , 10.1038/NBT1198
Martin Fussenegger, Stefan Schlatter, Daniel Dätwyler, Xenia Mazur, James E. Bailey, Controlled proliferation by multigene metabolic engineering enhances the productivity of Chinese hamster ovary cells Nature Biotechnology. ,vol. 16, pp. 468- 472 ,(1998) , 10.1038/NBT0598-468
Emanuele Cozzi, David J.G. White, The generation of transgenic pigs as potential organ donors for humans. Nature Medicine. ,vol. 1, pp. 964- 966 ,(1995) , 10.1038/NM0995-964