Flow cytometry for the assessment of animal sperm integrity and functionality: state of the art.

摘要: Flow cytometry is now a recognized methodology within animal spermatology, and has moved from being research tool to become routine in the assessment of semen destined breeding. The availability 'bench-top' flow cytometers newer versatile markers for cell structure function had allowed instrumentation measure more sperm parameters, viability reactiveness when exposed exogenous stimuli, increase our capabilities sort spermatozoa potential fertilizing capacity, or chromosomal sex. present review summarizes state art regarding applied andrology, albeit keeping an open comparative intent. It critically evaluates future diagnostics fertility development current reproductive technologies such as freezing, selection sorting. methods will probably further revolutionize understanding physiology their functionality, undoubtedly extend its application isolating many uncharacterized features spermatozoa. However, continuous follow-up necessity owing technical developments complexity mapping