Decreased dopamine D4 receptor expression increases extracellular glutamate and alters its regulation in mouse striatum.

摘要: To better understand the effect of dopamine D4 receptor (DRD4) on glutamate (Glu) neurotransmission in brain, we utilized transgenic mice with partial or complete removal functional DRD4 plasma membrane expression (DRD4+/− and DRD4−/−, respectively). We measured resting extracellular Glu levels, clearance kinetics, KCl-evoked release striatum nucleus accumbens core these using vivo amperometry coupled to a novel microelectrode array configured for sub-second detection Glu. Recordings from DRD4−/− DRD4+/− were compared their wild-type littermates (DRD4+/+). Resting levels increased (p<0.01). kinetics significantly decreased dorsal (p<0.05). overflow was reliably but unchanged three groups. By contrast, no changes Glu, uptake observed among genotypes. These data indicate that is involved modulation neurotransmission, primarily striatum. A understanding control by may improve our physiological role disorders such as attention-deficit/hyperactivity disorder schizophrenia.