Cigarette smoking induces only marginal changes in sperm DNA methylation levels of patients undergoing intracytoplasmic sperm injection treatment.

摘要: DNA methylation plays important roles in genome stability and regulation of gene expression. This study was designed to determine the influence cigarette smoking on sperm methylation. From a genome-wide survey samples, differentially methylated target CpGs should be selected subjected local deep bisulphite sequencing. Obtained data are compared parameters (ICSI) outcome. Similar pilot study, samples were Infinium 450K BeadChip arrays identify alterations between smokers nonsmokers males. Routine testing significantly altered CpG site performed more using Of approximately 485,000 sites analysed, only seven found show significant difference >20% with top six overlapping common SNP sites. The remaining (cg19455396) is located intron 12 TAP2 gene. results sequencing showed tendency towards hypomethylation group. could not detect biologically relevant positions that beyond individual-specific effects may caused by other environmental factors.