Three-Dimensional Construction of a Rabbit Anterior Corneal Replacement for Lamellar Keratoplasty.

摘要: The aim of this study was to construct a rabbit anterior corneal replacement for transplantation using acellular porcine matrix (APCM) and epithelial or stromal cells. APCM prepared from fresh cornea treated with 0.5% (wt./vol.) sodium dodecyl sulfate (SDS) solution. expanded cells were first injected into parallel its surface cultured in shaking culture system 7 days obtain the construct. Next, on another lamella. had phenotype similar that normal cornea, high expression cytokeratin 3 cell layer vimentin More importantly, integrated well implanted host tissue, implant maintained transparency 6-month follow-up, although there slight haze central area. endothelium surgery density mosaic pattern as shown by confocal laser microscopy, regenerated showed morphology natural These results demonstrate constructed exhibits an excellent biological property lamellar keratoplasty might be possible alternative human tissue future.